Xenograft Image Analysis Presentation, Boston 2013

Earlier this year, OracleBio’s Chief Scientific Officer, Dr. Lorcan Sherry gave a presentation entitled  ’Analysis of Drug Response Read-Outs in Xenograft Tissue using Definiens Tissue Studio’  at the 4th International Definiens Symposium in Boston, MA  His presentation highlighted some of OracleBio’s experiences in using Definiens Tissue Studio for evaluation of common Xenograft drug response read-outs. These included evaluation […] Read more »

Image Analysis Applications in Drug Discovery

Quantification of histomorphometry and IHC marker staining read outs within cells and tissues is an essential read out across many Pharmaceutical R&D studies. Continued advances in digital image analysis software, in combination with whole slide imaging, now allows for highly accurate, objective and rapid assessment of marker staining across whole tissue sections on a cell […] Read more »

FDA issues draft guidance on the use of histology in biomarker qualification studies

In recent years there has been a significant increase in the use of histological approaches in the generation of data to support the characterisation, validation and qualification of diagnostic, prognostic and predictive biomarkers, to be used as in both the clinical and pre-clinical setting. In response to this increase, in December 2011 the Food and […] Read more »

Quantification of Beta Amyloid Plaques in Alzheimer’s disease by Definiens image analysis

Detection of Beta-Amyloid (Aβ) plaques in Alzheimer’s Disease (AD) using Immunohistochemistry (IHC) and image analysis was the subject of a recent publication in the Journal of Neuroscience Methods, 2011 (http://www.ncbi.nlm.nih.gov/pubmed/22019329) by Samaroo et al. at Pfizer. They applied a high throughput object-based image analysis methodology using Definiens software and validated it to quantify immunohistochemically stained β-Amyloid […] Read more »

Xenograft IHC image analysis and getting the most out of R&D studies

Pre-clinical Xenograft Tumor models coupled with immunohistochemistry (IHC) and quantitative image analysis is an established approach within many Oncology focused Pharmaceutical R&D programs to investigate factors involved in malignant transformation, invasion and metastasis, as well as to examine response to therapy. However, achieving robust, detailed and consistent results from study to study depends on a […] Read more »

Biomarker IHC & image analysis within R&D studies – A gold standard approach.

Traditional Histology and Immunohistochemistry (IHC) techniques are established methods for evaluating tissue parameters from a variety of target validation, efficacy and toxicity studies within pre-clinical drug R&D programs.  I spent many of my earlier years within the Pharmaceutical Industry performing Histology and IHC techniques as part of Cardiovascular target validation programs. Coupled with microscopy, these […] Read more »